Firmware upload needs totally reworked but this helps a bit for now.

pyad2usb/util.py

@@ -77,7 +77,7 @@ class Firmware(object):
                         if progress_callback is not None:
                             progress_callback(Firmware.STAGE_UPLOADING)
 
-                        time.sleep(0.05)
+                        time.sleep(0.0)
 
         def read_until(pattern, timeout=0.0):
             """
@@ -134,10 +134,12 @@ class Firmware(object):
                 stage_callback(Firmware.STAGE_WAITING)
                 time.sleep(1)
 
+        time.sleep(1)
+
         # Reboot the device and wait for the boot loader.
         stage_callback(Firmware.STAGE_BOOT)
         dev.write("=")
-        read_until('!boot', timeout=15.0)
+        read_until('......', timeout=15.0)
 
         # Get ourselves into the boot loader and wait for indication
         # that it's ready for the firmware upload.

test.py

@@ -75,14 +75,15 @@ def upload_usb():
     dev = pyad2usb.ad2usb.devices.USBDevice()
 
     dev.open(no_reader_thread=True)
-    pyad2usb.ad2usb.util.Firmware.upload(dev, 'tmp/ademcoemu_V2_2a_6.hex', handle_firmware)
+    #pyad2usb.ad2usb.util.Firmware.upload(dev, 'tmp/ademcoemu_V2_2a_6.hex', handle_firmware)
     dev.close()
 
 def upload_serial():
-    dev = pyad2usb.ad2usb.devices.SerialDevice(interface='/dev/ttyUSB2')
+    dev = pyad2usb.ad2usb.devices.SerialDevice(interface='/dev/ttyUSB0')
 
-    dev.open()
+    dev.open(no_reader_thread=True)
     pyad2usb.ad2usb.util.Firmware.upload(dev, 'tmp/ademcoemu_V2_2a_6.hex', handle_firmware)
+    #pyad2usb.ad2usb.util.Firmware.upload(dev, 'tmp/ademcoemu-V2-2a-5-beta20-C4.hex', handle_firmware)
     dev.close()
 
 def upload_usb_serial():